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Addgene inc tac gfp
Tac Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tac+gfp/WWP-Luc+(p21)+(Plasmid+%2316249)/pmc12402077-333-26-27
Average 92 stars, based on 8 article reviews
tac gfp - by Bioz Stars, 2026-10
92/100 stars

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Plasmid Preparation:

Article Title: Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3 ) amplified from TAC-GFP (Addgene #162494) and the FKBP fragment amplified from GFP-M7HMM-FKBP. .. A mouse harmonin b fragment (residues 296–728 of NM_01163733) with FRB at the N-terminus and EGFP at the C-terminus (i.e., FRB-DFCR-EGFP) was expressed using a plasmid constructed from pEGFP-N3 (Clontech), an FRB fragment amplified from pEGFP-FRB and a harmonin b fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.

Article Title: Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3) amplified from TAC-GFP (Addgene # 162494) and the FKBP fragment. .. A mouse USH1C fragment (residues 296–728 of NM_01163733) was expressed with FRB at the N-terminus and EGFP at the C-terminus using a plasmid constructed from pEGFP-N3 (Clonetech) inserting DNA fragment amplified from pEGFP-FRB and a USH1C fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.

Construct:

Article Title: Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3 ) amplified from TAC-GFP (Addgene #162494) and the FKBP fragment amplified from GFP-M7HMM-FKBP. .. A mouse harmonin b fragment (residues 296–728 of NM_01163733) with FRB at the N-terminus and EGFP at the C-terminus (i.e., FRB-DFCR-EGFP) was expressed using a plasmid constructed from pEGFP-N3 (Clontech), an FRB fragment amplified from pEGFP-FRB and a harmonin b fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.

Article Title: Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3) amplified from TAC-GFP (Addgene # 162494) and the FKBP fragment. .. A mouse USH1C fragment (residues 296–728 of NM_01163733) was expressed with FRB at the N-terminus and EGFP at the C-terminus using a plasmid constructed from pEGFP-N3 (Clonetech) inserting DNA fragment amplified from pEGFP-FRB and a USH1C fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.

Amplification:

Article Title: Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3 ) amplified from TAC-GFP (Addgene #162494) and the FKBP fragment amplified from GFP-M7HMM-FKBP. .. A mouse harmonin b fragment (residues 296–728 of NM_01163733) with FRB at the N-terminus and EGFP at the C-terminus (i.e., FRB-DFCR-EGFP) was expressed using a plasmid constructed from pEGFP-N3 (Clontech), an FRB fragment amplified from pEGFP-FRB and a harmonin b fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.

Article Title: Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells
Article Snippet: .. A plasmid to express IL2Rα-EGFP-FKBP was constructed from pEGFP-C1 inserting a DNA fragment encoding the Tac antigen (human IL2 receptor alpha subunit; NM_000417.3) amplified from TAC-GFP (Addgene # 162494) and the FKBP fragment. .. A mouse USH1C fragment (residues 296–728 of NM_01163733) was expressed with FRB at the N-terminus and EGFP at the C-terminus using a plasmid constructed from pEGFP-N3 (Clonetech) inserting DNA fragment amplified from pEGFP-FRB and a USH1C fragment amplified from the cDNA of USH1C isoform b4, a gift from Nicolas Grillet at Stanford University.



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